R绘图(6): 拯救初学者——发表级绘图全能包ggpubr_stat_compare_means 线加粗

今天花了很多时间整理这个包的绘图函数，不得不说这个基于ggplot2的包，是真的友好，很适合初学者。可能对于熟悉ggplot2的人来说，ggpubr的存在有些多余，但这并不妨碍它成为一个优秀的R包。

接下来我主要依据变量类型，对这个包的十来种函数，近30种图形进行展示，几乎涵盖了平常看到的大多数图。这篇推文也很可能成为你见过的最详细的ggpubr中文教程。 公众号后台回复20210330，获取今天的代码和图形示例pdf。

install.packages("ggpubr")
library(ggpubr)
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1. 单变量——连续型

1.1 密度图

#创建数据框
set.seed(0330)
mydata = data.frame(
  group = rep(c("A", "B","C","D"), each=200),
  value = c(rnorm(200, mean = 2), rnorm(200, 6),rnorm(200,2,4),rnorm(200,6,4))
)

ggdensity(mydata, x = "value", y="..density..", #或者"..count.."，默认为"..density.."
          fill = "lightgray",
          add = "mean", #或者"median",
          rug = TRUE #在图形下方添加密度线
          )
ggsave("density1.pdf",width = 10,height = 10,units = "cm")
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分组绘制

ggdensity(mydata, x = "value",
          color = "group", fill="group", #分组
          palette = "Dark2",#或者"aaas"这种ggsci包的配色形式,或者"#00AFBB"这种编码形式
          add = "mean",
          rug = TRUE,
          alpha=0.2, #调整透明度
          xlab=F,ylab = "Density",
          facet.by="group", #分面
          panel.labs=list(group = c("1", "2", "3", "4")), #修改每个panel的名字
          title="density plot",
          ggtheme=theme_bw() #可以是ggplot2中的主题类型
)
ggsave("density2.pdf",width = 13,height = 12,units = "cm")
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1.2 密度图叠加正态分布

set.seed(0330)
mydata = data.frame(
  group = rep(c("A", "B"), each=200),
  value = c(rnorm(200, 2), rnorm(200, 6, 4))
)

ggdensity(mydata, x = "value", fill = "red") +
  stat_overlay_normal_density(color = "red", linetype = "dashed")+
  scale_x_continuous(limits = c(-5,20))
ggsave("density3.pdf",width = 10,height = 10,units = "cm")
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分组绘制

ggdensity(mydata, x = "value", fill = "group") +
  stat_overlay_normal_density(aes(color=group), linetype = "dashed")+
  scale_x_continuous(limits = c(-5,20))
ggsave("density4.pdf",width = 10,height = 10,units = "cm")
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分组+分面

ggdensity(mydata, x = "value", fill = "group", facet.by = "group") +
  stat_overlay_normal_density(aes(color=group), linetype = "dashed")+
  scale_x_continuous(limits = c(-5,20))
ggsave("density5.pdf",width = 16,height = 10,units = "cm")
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1.3 直方图

gghistogram(mydata, x = "value", fill = "lightgray",
            add = "mean", rug = TRUE)
ggsave("hist1.pdf",width = 10,height = 10,units = "cm")
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分组绘制

gghistogram(mydata, x = "value", fill = "group",
            add = "mean", rug = TRUE,
            palette = c("#00AFBB", "#E7B800"))
ggsave("hist2.pdf",width = 10,height = 10,units = "cm")
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添加核密度图

gghistogram(mydata, x = "value", fill = "group",
            rug = TRUE,
            palette = c("#00AFBB", "#E7B800"),
            add_density = TRUE)
ggsave("hist3.pdf",width = 10,height = 10,units = "cm")
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2. 双变量——x离散，y连续

2.1 箱型图

library(patchwork)
set.seed(0330)
mydata = data.frame(
  group = rep(c("A", "B"), each=100),
  group2 = rep(c("g1","g2","g1","g2"),each=50),
  value = c(rnorm(100, 2), rnorm(100, 6, 4))
)

#下面的加号表示拼接图形
ggboxplot(mydata, x = "group", y = "value", width = 0.8)+
ggboxplot(mydata, x = "group", y = "value", width = 0.8, orientation = "horizontal")+
ggboxplot(mydata, x = "group", y = "value", width = 0.8, notch = TRUE,order = c("B","A"))+
ggboxplot(mydata, x = "group", y = "value", width = 0.8, select = c("A"))
ggsave("box1.pdf",width = 10,height = 10,units = "cm")
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orientation调整图形方向；notch添加缺口；order调整顺序；select选择特定的水平来画图

ggboxplot(mydata, x = "group", y = "value", width = 0.8, add = "jitter",add.params=list(color = "lightblue",size=1, shape = 17))+
ggboxplot(mydata, x = "group", y = "value", width = 0.8, add = "dotplot",add.params=list(color = "lightblue",size=0.5))
ggsave("box2.pdf",width = 16,height = 10,units = "cm")
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上面的add添加额外图形 add.params对附加图形的参数进行调整，shape表示点的形状，可以参加下图

内部分组

ggboxplot(mydata, x = "group", y = "value", width = 0.6, color = "black",fill="group2",palette = c("#00AFBB", "#E7B800"),
          xlab = F, #不显示x轴的label
          bxp.errorbar=T,bxp.errorbar.width=0.4, #添加errorbar
          size=1, #箱型图边线的粗细
          outlier.shape=NA, #不显示outlier
          legend = "right") #图例放右边
ggsave("box3.pdf",width = 10,height = 10,units = "cm")
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2.1.1 箱型图添加配对连线

可以接受两种数据框

mydata2=mydata
mydata2$group2=NULL
head(mydata2)
# group    value
# 1     A 3.551687
# 2     A 3.664068
# 3     A 2.194454
# 4     A 2.569605
# 5     A 2.579997
# 6     A 1.837967
ggpaired(mydata2, x = "group", y = "value",
         color = "group", line.color = "gray", line.size = 0.4,
         palette = "npg")

mydata2$id=rep(1:100,2)
mydata2=mydata2%>%reshape2::dcast(id~group)
head(mydata2)
# id        A         B
# 1  1 3.551687  4.720074
# 2  2 3.664068  7.821049
# 3  3 2.194454  8.956841
# 4  4 2.569605 -4.450063
# 5  5 2.579997  7.568216
# 6  6 1.837967  5.133688
ggpaired(mydata2, cond1 = "A", cond2 = "B",
         color = "condition", line.color = "gray", line.size = 0.4,
         palette = "npg")
ggsave("box4.pdf",width = 10,height = 10,units = "cm")
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上面两种数据框得到的图是一模一样的

2.1.2 添加p值

一般用法

ggboxplot(mydata, x = "group", y = "value", width = 0.8, 
          add = "dotplot",add.params=list(color = "lightblue",size=0.5))+
  stat_compare_means(method = "t.test")
ggsave("box5.pdf",width = 10,height = 10,units = "cm")
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成对数据

ggpaired(mydata2, cond1 = "A", cond2 = "B",
         color = "condition", line.color = "gray", line.size = 0.4,
         palette = "npg")+
  stat_compare_means(paired = TRUE)
ggsave("box6.pdf",width = 10,height = 10,units = "cm")
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多于两个组时，定义想检验的配对

my_comparisons <- list( c("0.5", "1"), c("1", "2"), c("0.5", "2") )
ggboxplot(ToothGrowth, x = "dose", y = "len",
          color = "dose", palette = "npg")+
  #两两比较的p值
  stat_compare_means(comparisons = my_comparisons, label.y = c(29, 35, 40))+
  #整体的p值
  stat_compare_means(label.y = 45)
ggsave("box7.pdf",width = 10,height = 10,units = "cm")
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固定某一组，其他组与其比较

ggboxplot(ToothGrowth, x = "dose", y = "len",
          color = "dose", palette = "npg")+
  # 整体的p值
  stat_compare_means(method = "anova", label.y = 40)+ 
  #label中用点表示显著性
  stat_compare_means(aes(label = ..p.signif..),
                     method = "t.test", ref.group = "0.5")
ggsave("box8.pdf",width = 10,height = 10,units = "cm")
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分组/分面之后再做比较

ggboxplot(ToothGrowth, x = "supp", y = "len",
          color = "supp", palette = "npg",
          add = "jitter",
          facet.by = "dose")+
  #label中去掉检验方法
  stat_compare_means(aes(label = paste0("p = ", ..p.format..)))
ggsave("box9.pdf",width = 10,height = 10,units = "cm")
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2.2 小提琴图

整体上参数选项和箱型图差不多

ggviolin(mydata, x = "group", y = "value", fill = "group",
         palette = c("#00AFBB", "#E7B800"),
         add = "boxplot", add.params = list(fill = "white"))+
ggviolin(mydata, x = "group", y = "value", color = "group2", #内部分组
         palette = c("#00AFBB", "#E7B800"), 
         add = "boxplot")
ggsave("violin1.pdf",width = 16,height = 10,units = "cm")
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2.3 柱形图

2.3.1 数据已经统计好

df1 <- data.frame(group=c("A", "B", "C"),
                 len=c(6, 10, 14))
ggbarplot(df1, "group", "len",
          fill = "group", color = "group",
          palette = c("#00AFBB", "#E7B800", "#FC4E07"),
          label = TRUE, lab.pos = "in", lab.col = "white")
ggsave("bar1.pdf",width = 10,height = 10,units = "cm")
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2.3.2 数据没有统计好

(需要函数帮你计算，比如组内求和，一般反映在纵坐标上，这是函数帮你算的)

df2 <- data.frame(group=rep(c("A", "B", "C"),2),
                  group2=rep(c("1", "2"), each=3),
                  len=c(6, 15, 3, 4, 10, 5))
# group group2 len
# 1     A      1   6
# 2     B      1  15
# 3     C      1   3
# 4     A      2   4
# 5     B      2  10
# 6     C      2   5
ggbarplot(df2, "group", "len",
          fill = "group2", color = "group2", palette = "Paired",
          label = TRUE, lab.col = "white", lab.pos = "in")+
ggbarplot(df2, "group", "len",
          fill = "group2", color = "group2", palette = "Paired",
          label = TRUE,
          position = position_dodge(0.9)) #范围0-1，表示柱子之间的错开程度
ggsave("bar2.pdf",width = 16,height = 10,units = "cm")
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2.3.3 添加误差棒

df3 <- mydata
#这时累加
ggbarplot(df3, x = "group", y = "value")+
#这时求均值
ggbarplot(df3, x = "group", y = "value",
          add = "mean")+
#添加误差棒,error.plot选择展示形式，默认上下都展示
ggbarplot(df3, x = "group", y = "value",
          add = "mean_se",
          error.plot = "upper_errorbar")+
#内部分组
ggbarplot(df3, x = "group", y = "value", color = "group2", 
          add = "mean_se", palette = c("#00AFBB", "#E7B800"),
          position = position_dodge())
ggsave("bar3.pdf",width = 16,height = 16,units = "cm")
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2.4 连线图

#数据计算好，可以直接用
ggline(df1, x = "group", y = "len")+
#内部分组，点线的形状和颜色均不同
ggline(df2, x = "group", y = "len", 
       linetype = "group2", shape = "group2",#点的形状
       color = "group2", palette = c("#00AFBB", "#E7B800"))+
#添加点和误差棒
ggline(df3, x = "group", y = "value",
       add = c("mean_se","dotplot"),add.params = list(size=0.5),
       color = "steelblue")+
#内部分组，线的颜色不一样
ggline(df3, x = "group", y = "value", color = "group2",
       add = "mean_se", palette = c("#00AFBB", "#E7B800"))
ggsave("line1.pdf",width = 16,height = 16,units = "cm")
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2.5 饼图

df1$ratio=paste(df1$group,"(",round(df1$len / sum(df1$len),3) * 100,"%)",sep = "")
ggpie(df1, "len", label = "ratio",
      fill = "group", color = "white",
      palette = c("#00AFBB", "#E7B800", "#FC4E07"))+
ggpie(df1, "len", label = "ratio",
      lab.pos = "in", lab.font = "white",
      fill = "group", color = "white",
      palette = c("#00AFBB", "#E7B800", "#FC4E07"))
ggsave("pie1.pdf",width = 16,height = 10,units = "cm")
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label添加文本注释； color是扇形的边线，lab.pos调整文本的位置，lab.font调整文本字体颜色

2.6 圆环图

ggdonutchart(df1, "len", label = "ratio",
             lab.pos = "in", lab.font = "white",
             fill = "group", color = "white",
             palette = c("#00AFBB", "#E7B800", "#FC4E07"))
ggsave("donut1.pdf",width = 10,height = 10,units = "cm")
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2.7 克利夫兰点图

ggdotchart(df2, x = "group", y = "len",
           color = "group2", size = 3,
           add = "segment",
           add.params = list(color = "lightgray", size = 1.5),
           position = position_dodge(0.5),
           palette = "jco",
           ggtheme = theme_pubclean())
ggsave("Clevelands_Dot1.pdf",width = 10,height = 10,units = "cm")
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3. 双变量——x, y都连续

3.1 散点图添加回归线，相关系数

df4=mtcars
df4$cyl=as.factor(df4$cyl)
ggscatter(df4, x = "wt", y = "mpg",
          color = "black", size = 3, # 点的颜色，大小
          add = "reg.line",  # 添加回归线
          add.params = list(color = "blue", fill = "lightgray"), # 回归线的调整
          conf.int = TRUE, # 回归线的置信区间
          cor.coef = TRUE, # 添加相关系数
          cor.coeff.args = list(method = "pearson", label.x = 3, label.sep = "\n")#相关系数的调整
)
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3.2 分组计算相关系数

ggscatter(df4, x = "wt", y = "mpg",
          color = "cyl", palette = "jco",
          add = "reg.line", conf.int = TRUE)+
  stat_cor(aes(color = cyl), label.x = 3)
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3.3 局部回归

ggscatter(df4, x = "wt", y = "mpg",
          add = "loess", conf.int = TRUE)
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3.4 添加分组椭圆，均值点，以及辐射线

ggscatter(df4, x = "wt", y = "mpg",
          color = "cyl", shape = "cyl",
          palette = c("#00AFBB", "#E7B800", "#FC4E07"),
          ellipse = TRUE, mean.point = TRUE,
          star.plot = TRUE)
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3.5 添加文本注释

df4$name <- rownames(df4)
ggscatter(df4, x = "wt", y = "mpg",
          color = "cyl", palette = c("#00AFBB", "#E7B800", "#FC4E07"),
          label = "name", repel = TRUE)+plot_layout(widths = c(1,2))
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3.6 散点图边缘添加密度图/箱型图

ggscatterhist(
  iris, x = "Sepal.Length", y = "Sepal.Width",
  color = "Species", size = 3, alpha = 0.6,
  palette = c("#00AFBB", "#E7B800", "#FC4E07"),
  margin.params = list(fill = "Species", color = "black", size = 0.2)
)
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ggscatterhist(
  iris, x = "Sepal.Length", y = "Sepal.Width",
  color = "Species", size = 3, alpha = 0.6,
  palette = c("#00AFBB", "#E7B800", "#FC4E07"),
  margin.plot = "boxplot",
  ggtheme = theme_bw()
)
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感谢你能读到这里，有任何疑问欢迎后台留言。

因水平有限，有错误的地方，欢迎批评指正！