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knitr::opts_chunk$set(message = FALSE, warning = FALSE)
library(tidyverse)
library(patchwork)
library(ggpubr)
library(rstatix)
# rm(list = ls())
options(stringsAsFactors = F)
options(future.globals.maxSize = 10000 * 1024^2)
inputdata <- data.frame(
GroupA = c(rnorm(80, 4, 2), rnorm(120, 10, 3)),
GroupB = c(rnorm(130, 4, 2), rnorm(70, 10, 3)))
plotdata <- inputdata %>%
tidyr::pivot_longer(
cols = GroupA:GroupB,
names_to = "variable",
values_to = "value")
head(plotdata)
stat.test <- plotdata %>%
t_test(value ~ variable) %>%
adjust_pvalue(method = "bonferroni") %>%
add_significance("p.adj")
stat.test
绘制基础的直方图
p1 <- plotdata %>% dplyr::filter(variable == "GroupA") %>% ggplot(aes(x = value)) + geom_histogram(binwidth = 1, color = "black", fill = "#03B0AB", cex = 1) + geom_density(aes(value, y = after_stat(count)), color = "black", fill = "#03B0AB", alpha = 0.2, cex = 1) + scale_x_continuous(expand = c(0, 0)) + scale_y_continuous(expand = c(0, 0), limits = c(0, 33)) + labs(x = NULL, y = "Count") + theme_classic(base_size = 15) + theme(axis.text = element_text(color = "black")) p2 <- plotdata %>% dplyr::filter(variable == "GroupB") %>% ggplot(aes(x = value)) + geom_histogram(binwidth = 1, color = "black", fill = "#FB632E", cex = 1) + geom_density(aes(value, y = after_stat(count)), color = "black", fill = "#FB632E", alpha = 0.2, cex = 1) + scale_x_continuous(expand = c(0, 0)) + scale_y_continuous(expand = c(0, 0), limits = c(0, 33)) + labs(x = NULL, y = NULL) + theme_classic(base_size = 15) + theme(axis.line.y = element_blank(), axis.text.y = element_blank(), axis.ticks.y = element_blank(), axis.text = element_text(color = "black"))
pl <- p1 + geom_rect(aes(xmin = 12, xmax = 16, ymin = mean(inputdata$GroupA) + 3 * sd(inputdata$GroupA), ymax = mean(inputdata$GroupA) + 3.5 * sd(inputdata$GroupA)), color = "#03B0AB", fill = "transparent", cex = 1) + annotate("text", x = 13.5 , y = mean(inputdata$GroupA) + 3.2 * sd(inputdata$GroupA), label = "GroupA", size = 3.5) + annotate("segment", x = mean(inputdata$GroupA), xend = mean(inputdata$GroupA), y = 0, yend = 31, linetype = 2, cex = 1) + coord_cartesian(clip = "off", xlim = c(ceiling(min(inputdata$GroupA)) - 1, ceiling(max(inputdata$GroupA)) + 1)) + theme(axis.title = element_text(size = 11, face = "bold"), axis.text = element_text(color = "black", size = 9)) + p2 + geom_rect(aes(xmin = 12, xmax = 16, ymin = mean(inputdata$GroupB) + 3 * sd(inputdata$GroupB), ymax = mean(inputdata$GroupB) + 3.5 * sd(inputdata$GroupB)), color = "#FB632E", fill = "transparent", cex = 1) + annotate("text",x = 13.5, y = mean(inputdata$GroupB) + 3.2 * sd(inputdata$GroupB), label = "GroupB", size = 3.5) + annotate("segment", x = mean(inputdata$GroupB), xend = mean(inputdata$GroupB), y = 0, yend = 31, linetype = 2, cex = 1) + xlab("Value") + theme(axis.title.x = element_text(hjust = -0.2)) + coord_cartesian(clip = "off", xlim = c(ceiling(min(inputdata$GroupB)) - 1, ceiling(max(inputdata$GroupB)) + 1)) + annotate("segment", x = -2*mean(inputdata$GroupA), xend = mean(inputdata$GroupB), y = 31, yend = 31, cex = 1) + annotate("text", x = -mean(inputdata$GroupA)+2, y = 32, size = 5, label = paste0("p=", stat.test$p.adj)) + theme(axis.title = element_text(size = 11, face = "bold"), axis.text = element_text(color = "black", size = 9)) pl
devtools::session_info()
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